Composite

Part:BBa_K798019:Design

Designed by: Chao Zhang   Group: iGEM12_Fudan_D   (2012-09-25)


ADH4 homologous arm-TRP1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 624
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 624
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

In order to improve the success rate of recombination, the homologous arm is 60bp.


Source

ADH4 homologous arm was directly synthetized while TRP1 was amplified from the yeast genome by PCR.

References